Bioprocessing: an exhilarating new engineering self-discipline. It combines the improvement and optimization of biotechnological procedures with powerful thoughts to get better and purify the specified items. security in addition to fee play an immense position here.
This quantity covers the immensely differentiated spectrum of suggestions and operations of bioprocessing, offered through the main powerfuble specialists within the box. an outline of upstream and downstream processing is given, fermentation and phone tradition methods and the layout of microbial fermenters are provided. A last workforce of chapters is devoted to problems with approach validation, size, and regulation.
subject matters incorporated are: business telephone Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen move/ Scale Implications/ Fermentation facts research/ telephone and particles removing/ Protein Purification/ Electrokinetic Separations/ ultimate restoration Steps/ method Validation
Chapter 1 Fermentation: an outline (pages 7–22): Barry C. Buckland and Malcolm D. Lilly
Chapter 2 commercial Animal phone tradition (pages 23–38): Brian D. Kelley, Tzyy?Wen Chiou, Morris Rosenberg and Daniel I. C. Wang
Chapter three evaluation of Downstream Processing (pages 39–55): Ron Spears
Chapter four Proteins and Peptides as medicines: assets and strategies of Purification (pages 57–73): Stuart E. Builder, Robert L. Garnick, James C. Hodgdon and John R. Ogez
Chapter five Bioreactors: Description and Modelling (pages 77–104): Jens Nielsen and John Villadsen
Chapter 6 phone tradition Bioreactors (pages 105–126): Athanassios Sambanis and Wei?Shou Hu
Chapter 7 Media for Microbial Fermentations (pages 127–139): Randolph L. Greasham
Chapter eight Media for mobilephone tradition (pages 141–156): R. A. Wove
Chapter nine Media and Air Sterilization (pages 157–184): Gokaraju okay. Raju and Charles L. Cooney
Chapter 10 Oxygen move and combining: Scale?Up Implications (pages 185–217): Matthias Reuss
Chapter eleven Aeration in cellphone tradition Bioreactors (pages 219–281): John G. Aunins and Hans?Jurgen Henzler
Chapter 12 suggestions for Fermentation with Recombinant Organisms (pages 283–294): Tadayuki Imanaka
Chapter thirteen Anaerobic Fermentations (pages 295–318): Larry E. Erickson, Daniel Y. C. Fung and Pravate Tuitemwong
Chapter 14 Fermentation tracking and keep watch over (pages 319–354): Thomas Chattaway, Gary A. Montague and A. Julian Morris
Chapter 15 Fermentation facts research for prognosis and keep an eye on (pages 355–400): Gregory Stephanopoulos, Konstantin Konstantinov, Urs Saner and Toshiomi Yoshida
Chapter sixteen layout of Aseptic, Aerated Fermentors (pages 401–426): Marvin Charles and Jack Wilson
Chapter 17 Biotransformations and Enzyme Reactors (pages 427–466): Andreas S. Bommarius
Chapter 18 phone and mobilephone particles elimination: Centrif Ugation and Crossflow Filtration (pages 469–503): Rajiv V. Datar and Carl?Gustaf Rosen
Chapter 19 telephone Disruption and Isolation of Non?Secreted items (pages 505–526): Horst Schutte and Maria?Regina Kula
Chapter 20 In vitro Protein Refolding (pages 527–555): Jeffrey L. Cleland and Daniel I. C. Wang
Chapter 21 Liquid?Liquid Extraction (Small Molecules) (pages 557–592): Karl Schugerl
Chapter 22 Protein Purification by way of Liquid?Liquid Extraction (pages 593–616): Brian D. Kelley and T. Alan Hatton
Chapter 23 Protein Separation and Purification (pages 617–642): Jan?Christer Janson and Lars Ryden
Chapter 24 Affinity Separations (pages 643–677): Srikanth Sundaram and Martin L. Yarmush
Chapter 25 Electrokinetic Separations (pages 679–693): Alan J. Grodzinsky and Martin L. Yarmush
Chapter 26 ultimate restoration Steps: Lyophilization, Spray?Drying (pages 695–714): Christian F. Golker
Chapter 27 Analytical Protein Chemistry (pages 717–737): Srikanth Sundaram, David M. Yarmush and Martin L. Yarmush
Chapter 28 Biotechnology Facility layout and procedure Validation (pages 739–767): Michael G. Beatrice
Chapter 29 remedy of organic Waste (pages 769–787): Daniel F. Liberman
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Additional info for Biotechnology: Bioprocessing, Volume 3, Second Edition
CHO cells have been shown to undergo a reduction in gene copy number, if selection pressure is released (KAUFMAN, 1990a). While batch and fed-batch operation is of short enough duration to avoid stability problems, long-term continuous cultures may have more severe problems. Better analytical methods for animal cell genetic make up and copy number will need to be developed, as well as stable, high copy number expression systems. 2 Media Formulation The medium used to culture cells can have an enormous impact on maximum cell density, protein concentration, and ease of product recovery.
As the time pressure to bring a product to market is obvious, any delay incurred from implementing new technologies often becomes critical in the final analysis. 3 Current Applications sities are similar to those found in the homogeneous perfusion bioreactors. An important point often overlooked is that while perfusion systems operate at much higher cell densities than batch bioreactors and thus require smaller fermentors, they still require as much if not more ancillary equipment to support the continuous operation.
B. (1990), Controlling bacteriophage niques for mixing and mass transfer in bioreacinfections in industrial bioprocesses, Adv. Biotors, in: Harnessing Biotechnology f o r the 2lst chem. Eng. 43, 1-10. M. ), pp. REDA,K. , TH IENM. , FEYGIN,I. , MARCIN, Century (LADISCH, C. , CHARTRAIN, M. , GREASHAM,R. L. 178-182, Washington DC: American Chemical Society. (1991), Automatic whole broth multi-fermenter MANFRFDINI, sampling, J. Znd. Microbiol. 7, 215-220. , , SMITH,J . , LILLY,M. , Fox, R. I. (1990), The DONATI,G.