By Tom Waigh
This ebook provides the basics of molecular biophysics, and highlights the relationship among molecules and organic phenomena, making it an immense textual content throughout quite a few technological know-how disciplines.
The subject matters coated within the booklet include:
* section transitions that take place in biosystems (protein crystallisation, globule-coil transition etc)
* Liquid crystallinity to illustrate of the fragile diversity of in part ordered stages chanced on with organic molecules
* How molecules movement and propel themselves on the mobile level
* the overall good points of self-assembly with examples from proteins
* The part behaviour of DNA
The actual toolbox awarded inside this article is going to shape a foundation for college students to go into right into a wide variety of natural and utilized bioengineering fields in clinical, nutrients and pharmaceutical components.
Read Online or Download Applied Biophysics: A Molecular Approach for Physical Scientists PDF
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Extra resources for Applied Biophysics: A Molecular Approach for Physical Scientists
DNA has a number of interesting features with respect to its polymer physics. The persistence length (lp) of DNA is in the order of 50 nm for E. ). The large size of DNA has a number of important consequences; single fluorescently labelled DNA molecules are visible under an optical microscope, which proves very useful for high resolution experiments, and the cell has to solve a tricky packaging problem in vivo of how to fit the DNA inside the nucleus of a cell which is, at most, a few microns in diameter (it uses chromosomes).
Algebraically the potential ðVðrÞ as a function of particle separation (r)) is given by 64kTcÃ0 G20 Àkr VðrÞ A121 ¼À e þ B 12pr2 k ð2:18Þ where A121 is the Hamaker constant for the Van der Waals force, kÀ1 is the Debye screening length, cÃ0 is the bulk salt concentration, G0 is defined as tan (zqc=4kT), z is the valence of the particles, q is the electronic charge, kT is the thermal energy, B is the surface area of the particle, and c is the surface potential. 18) is due to the Van der Waals interaction and the second is from the screened electrostatic potential.
The addition of the polymer lowers the solvent’s chemical potential, and creates a depletion force that drives the colloidal surfaces together. 4). g. g. g. 25 mm silica spheres as a function of DNA concentration in (a) dilute and (b) semi-dilute DNA solutions measured with dual trap optical tweezers [Reprinted with permission from R. C. Crocker, T. C. G. Yodh, Macromolecules, 33, 177–186, Copyright (2000) American Chemical Society] 44 MESOSCOPIC FORCES For a dilute concentration of polymers in a colloidal solution the osmotic pressure (P) is proportional to the number density of polymer chains (N=V), with each chain contributing kT to the osmotic pressure: P¼ N kT V ð2:34Þ This pressure is analogous to that of an ideal gas, that gives rise to the Van der Waals equation (P ¼ NRT=V, where R is the ideal gas constant).