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Extra info for Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing: An introduction to the various fields of applications of the in vivo chlorophyll fluorescence also including the proceedings of the fir
300 ps and 550 ps show distinctly different excitation spectra. The corresponding PS II units have a different pigment composition in their antenna. At F the shorter-lived PS II component is associated with most of the o Chi alb protein complex as is again revealed from the pronounced excitation band at 650 nm. 2 a. ~ 3 2 flOX o ~ 670 680 690 700 620 710 640 660 680 700 Wavelength, nm Wavelength. nm (left): Decay-associated emission spectra for light-II-adapted cells of Scenedesmus (state II) at the F -level of fluorescence.
Often the resulting amplitudes A are plotted vs. A exc or A em I which is then called a decay-associated excitation or emission spectrum (DAS), respectively (Wendler and Holzwarth 1987, Holzwarth et al. 1987). The usefulness of such plots becomes apparent from eqn. 2 which provides the relationship between the amplitude Ai in a DAS and various molecular parameters of the antenne pigments. In many cases this relationship is simply given by (2) where C is a proportionality constant, exc is the excitation intensity, € exc the absorption coefficient at the excitation wavelength \xc' NChl the size of the antenna pool (number of Chis) giving rise to decay component i, F(A ) the normalized em fluorescence spectrum of this antenna pool and kra d the radiative lifetime of the pigments.
Pick, U. Hoffman-Falk, H. and Edelman, M. (1981) Proc. Natl. Acad. Sci. W. and Melis, A. (1987) Photochem. Photobiol. 46, 543-550 Mel is, A. (1984) J. Cell Biochem. 24, 271-285 Melis, A. (1985) Bi ochim. Bi ophys. Acta 808, 334-342 Mel is, A. M. (1983) Biochim. Biophys. Acta 724, 473-484 Melis, A. M. (1979) Photochem. Photobiol. 29,373-382 Melis, A. H. (1975) Photochem. Photobiol. 21, 431-437 Melis, A. H. (1976) Photochem. Photobiol. 23, 343-350 Melis, A. M. (1980) Biochim. Biophys. N. R. (1984) Biochim.