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By G. Heinrich Krause, Engelbert Weis (auth.), Hartmut K. Lichtenthaler (eds.)

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P. Mohanty, Phytomorphology, p. 262

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Extra info for Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing: An introduction to the various fields of applications of the in vivo chlorophyll fluorescence also including the proceedings of the fir

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300 ps and 550 ps show distinctly different excitation spectra. The corresponding PS II units have a different pigment composition in their antenna. At F the shorter-lived PS II component is associated with most of the o Chi alb protein complex as is again revealed from the pronounced excitation band at 650 nm. 2 a. ~ 3 2 flOX o ~ 670 680 690 700 620 710 640 660 680 700 Wavelength, nm Wavelength. nm (left): Decay-associated emission spectra for light-II-adapted cells of Scenedesmus (state II) at the F -level of fluorescence.

Often the resulting amplitudes A are plotted vs. A exc or A em I which is then called a decay-associated excitation or emission spectrum (DAS), respectively (Wendler and Holzwarth 1987, Holzwarth et al. 1987). The usefulness of such plots becomes apparent from eqn. 2 which provides the relationship between the amplitude Ai in a DAS and various molecular parameters of the antenne pigments. In many cases this relationship is simply given by (2) where C is a proportionality constant, exc is the excitation intensity, € exc the absorption coefficient at the excitation wavelength \xc' NChl the size of the antenna pool (number of Chis) giving rise to decay component i, F(A ) the normalized em fluorescence spectrum of this antenna pool and kra d the radiative lifetime of the pigments.

Pick, U. Hoffman-Falk, H. and Edelman, M. (1981) Proc. Natl. Acad. Sci. W. and Melis, A. (1987) Photochem. Photobiol. 46, 543-550 Mel is, A. (1984) J. Cell Biochem. 24, 271-285 Melis, A. (1985) Bi ochim. Bi ophys. Acta 808, 334-342 Mel is, A. M. (1983) Biochim. Biophys. Acta 724, 473-484 Melis, A. M. (1979) Photochem. Photobiol. 29,373-382 Melis, A. H. (1975) Photochem. Photobiol. 21, 431-437 Melis, A. H. (1976) Photochem. Photobiol. 23, 343-350 Melis, A. M. (1980) Biochim. Biophys. N. R. (1984) Biochim.

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